Prometheus
The new gold standard for precise characterization of protein stability
Prometheus utilizes a number of technologies (nanoDSF, DLS and Backreflection) to characterize thermal unfolding, particle sizing, and aggregation. Each one tells a different story about stability, and is used for a variety of applications. But they have one thing in common — they are all label-free.
NanoDSF
Measures thermal unfolding or chemical denaturation under native conditions and label-free by detecting changes in its intrinsic fluorescence during a thermal ramp or in the presence of a chemical denaturant
Measurement parameters: ratio: Tonset, Tm, ∆∆G, ∆G, C50, Ea, isothermal stability, reversibility of unfolding
Measurement parameters: ratio: Tonset, Tm, ∆∆G, ∆G, C50, Ea, isothermal stability, reversibility of unfolding
DLS
Detects molecule aggregates to determine the size of proteins over a wide concentration range
Measurement parameters: Tscattering, Tsize, rH, PDI, kD, D0
Measurement parameters: Tscattering, Tsize, rH, PDI, kD, D0
Backreflection
Determines aggregation status by measuring light intensity loss due to scattering
Measurement parameters: Tturbidity, reversibility of unfolding
Measurement parameters: Tturbidity, reversibility of unfolding
Dual UV system330 nm and 350 nm fluorescence
Wide concentration range5ug/ml to more than 250mg/ml
ReproducibilityPrecise, high-resolution data
Broad temperature range15 to 95 degree
Capillary technologiesFree of fluidics, no regular maintenance
Fast screeningMeasure 48 capillaries in 7 seconds and see more unfolding transitions
